A pilot study integrating absolute bacterial quantification into 16S amplicon sequencing of the urinary microbiota

This study aimed to include the culture independent absolute bacterial quantification into the workflow of 16S amplicon analysis of the human urinary microbiota. While this microbiota is widely acknowledged as sparse, the actual quantification attempts are rare. This is especially important in light of continuing debate on validity of sampling procedures and on the very existence of urinary bladder microbiota in humans.