Lactose operon sequences from: Spatial-scale evolutionary bias sheds light on the latitudinal diversity gradient

We placed the ancestral strain lac-ancestor of Escherichia coli K-12 GM4792 in two types of plates (Blue-plate and White-plate) for experimental evolution, with 24 replicates in each group. Both types of plates contained liquid medium and semi-solid medium sections. After 13 days of evolution, we randomly selected one single clone from both the liquid and semi-solid media of the 24 Blue plates for sequencing, and also sequenced one single clone from the semi-solid medium of the 24 White-plates, for a total of 72 single clones sequenced. We sequenced a gene fragment in the lactose operon region. The data show the sequence alignment of these 72 single clones. Additionally, we also included the gene sequences of the lac-ancestor and Escherichia coli K-12 MG1655.