RNA-Seq in kidneys from mice retro-orbitally injected with Lenti-miR-9 to determine the global gene-expression and potential mechanisms involved in the miR-9-5p-induced changes

To determine the global gene-expression and potential mechanisms involved in the miR-9-5p-induced changes during the UUO renal fibrosis model, RNA sequencing (RNA-Seq) analysis was performed in kidneys from mice retro-orbitally injected with 2x107 ifu/mouse of Lenti-miR-9 diluted in 30 µl saline serum 5 days before the UUO model (Group 3: Contralateral and Group 4: Obstructed kidneys). As controls, wild-type animals were provided with the same volume of Lenti-SC (Group 1: Contralateral and Group 2: Obstructed kidneys).