Activation of Xist by An Evolutionarily Conserved Function of the KDM5C Demethylase. Mus musculus

X-inactivation, which equalizes X-linked gene expression between the mammalian sexes, occurs due to the higher expression in females of the X-linked Kdm5c gene. Overall design: We performed RNA-Seq from mESC-derived EpiLCs. Libraries were depleted for rRNA and strand-specific according to the protocol described in PMID: 25607527. Day 2 differentiated EpiLCs were collected from 3 replicates each of 4 genotypes: female wild-type (fWT), female Kdm5c knock-out (fKO), male wild-type (mWT), and male Kdm5c knock-out (mKO)