MDA-PCa-2b cells, Control vs. ARlnc1 knockdown, AR-regulated gene signature generated by DHT treatment

(1) Transcription profiling of MDA-PCa-2b cells comparing ARlnc1 knockdown treated cells with control cells. Two methods were used to knockdown ARlnc1: siRNA or ASO. (2) Transcription profiling of MDA-PCa-2b cells comparing dihydrotestosterone (DHT) stimulated cells with vehicle treated cells. The goal is to determine AR-regulated gene expression signature in MDA-PCa-2b cells. (1) siRNA-mediated knockdown: cells transfected with 2 individual ARlnc1 siRNAs (si2 and si3), compared to non-targeting siRNA. (3 replicates each assay). (2) ASO-mediated knockdown: cells transfected with 2 individual ASOs targeting ARlnc1 (ASO2 and ASO6), compared to control ASO. (2 replicates each assay). (3) DHT-treated MDA-PCa-2b cells, compared to vehicle treated cells. (3 replicates)