The ERG-regulated LINC00920 promotes prostate cancer cell survival via the 14-3-3ε- FOXO pathway

LINC00920 is a tumor-associated lncRNA identified in the transcriptome dataset of the International Cancer Genome Consortium-Early Onset Prostate Cancer (ICGC-EOPC) cohort. SiRNA-mediated knockdown of LINC00920 negatively affected proliferation, colony formation, and migration of PC-3 prostate cancer cells. Genome-wide expression profiling was performed to identify cellular pathways affected by LINC00920. The effect of LINC00920 knockdown was determined in PC-3 prostate cancer cells. Two independent siRNAs were used to silence LINC00920, alongside a scrambled, non-targeting control. All transfection reactions were perfomed in 3 biological replicates. RNA isolation was performed 48 h post transfection.