Adaptive Gene Profiling of Mycobacterium Tuberculosis during Sub-lethal Kanamycin Exposure

Objectives: Mtb adaptation is the major threat to affective disease control.The objective of this study was to identify major contributors participating in Mtb adaptation process during sub-lethal drug exposure through next generation sequencing Methods: The sequence reads that passed quality filters were analyzed through Tophat-2 and HT-Seq after being unified with housekeeping genes expression level.Adaptation analysis was performed through classifying genes expression in count table by K-Means clustering algorithm and find interested genes pattern Results: Genes responsible for lipid metabolism strongly repressed while involved in virulence and information pathways up regulated under sub-lethal kanamycin stress.Moreover an adaptive network comprise of Ra1750,Ra3160,Ra3161,Ra2492 and Ra3893 identified in kanamycin treated Mtb Conclusions: Mtb has a complex regulatory inter-connected gene network supporting its survival under diverse environmental conditions. Overall design: H37Ra were cultured under sub-lethal kanamycin exposure along with untreated sample as control for 72 hours. Half of the bacteria were sub-cultured under same conditions and remaining were subjected for RNA Seq to compare adaptive gene profile of treated samples with control.