Expression data from small intestine crypts of Zfp148gt/+APCMin/+ and Zfp148+/+APCMin/+ mice

The transcription factor Zinc finger protein 148 (Zfp148) interacts physically with the tumor suppressor p53, but the siginficance of this interaction is not known. We recently showed that knockout of Zfp148 in mice leads to ectopic activation of p53 in tissues and cultured fibroblasts, suggesting that Zfp148 represses p53 activity. Here we hypothesized that targeting Zfp148 would unleash p53 activity and protect against cancer development, and test this idea in the APCMin/+ mouse model of intestinal adenomas. Crypt-enriched tissues were isolated by laser microdissection (PALM) from the small intestines (proximal) of Zfp148gt/+APCMin/+ and Zfp148+/+APCMin/+ mice for RNA extraction and hybridization to Affymetrix microarrays.转录因子锌指蛋白148（Zinc finger protein 148，Zfp148）可与肿瘤抑制因子p53发生物理相互作用，但该相互作用的生物学意义尚未明确。我们近期的研究证实，在小鼠体内敲除Zfp148会导致其组织及培养的成纤维细胞中出现p53的异位激活，这提示Zfp148可负调控p53的活性。本研究提出假说：靶向抑制Zfp148能够解除对p53活性的抑制，从而发挥抗肿瘤保护作用，并在肠道腺瘤模型APCMin/+小鼠中对该假说进行了验证。研究人员通过激光显微切割（laser microdissection，PALM）技术，从Zfp148gt/+APCMin/+与Zfp148+/+APCMin/+小鼠的近端小肠中分离得到富集隐窝的组织，用于RNA提取及Affymetrix基因芯片杂交实验。