Whole genome bisulfite sequencing

Male mice were subjected to frequent and mild scrotal heat stress (fmSHS，performed at 37℃ for 30 min every time, and repeated for 5 times) and control. This is a whole genome bisulfite sequencing of the DNA from these mice, which showed some differentially methylated region between control and fmSHS mice. The fmSHS sperm DNA revealed that DMRs overlapped 107 promoters (81 hypermethylated and 26 hypomethylated) and 622 gene body (dataset).. Three files (DMR_infotar.gz, mC_density.tar.gz, Methy_Info.tar.gz) were too big to load up. The main information on DMRs were collected in 2 excle tables.将雄性小鼠分为频繁轻度阴囊热应激（fmSHS，每次于37℃处理30分钟，重复5次）组与对照组。本数据集针对两组小鼠的DNA开展了全基因组亚硫酸氢盐测序（whole genome bisulfite sequencing），结果显示对照组与fmSHS组小鼠存在若干差异甲基化区域（differentially methylated region，DMR）。对fmSHS组小鼠精子DNA的分析表明，差异甲基化区域共重叠107个启动子区域（其中81个呈高甲基化、26个呈低甲基化）以及622个基因本体区域（gene body）。本次提供的文件中，DMR_infotar.gz、mC_density.tar.gz与Methy_Info.tar.gz三个文件因体积过大无法加载。差异甲基化区域的核心信息已整理于2张Excel表格中。