Transcriptional Reprogramming during Floral Fate Acquisition III

To gain insight into the auxin-dependent network during flower initiation, we used complementary RNAseq-based genomic analyses to obtain a global vision of the auxin-regulated gene network in the SAM before progressively converging on the identification of a high-confidence core network acting during the early phases of flower initiation. Overall design: To further define a high-confidence auxin-dependent network acting during flower initiation, we consolidated our SAM auxin response transcriptome by also profiling the transcriptome of an inducible repressor of auxin signaling (pRPS5A-GR-bdl (Weijers et al., 2006), Figure 1B) using inflorescence tissues treated with or without 10µM dexamethasone (dex) for 2 hours.