Effect of integrated stress response inhibitor (ISRIB) on eIF2a response and hippocampal-dependent behavioral outcomes in controlled cortical impact and closed head injury TBI male mouse models

STUDY PURPOSE: The purpose of the study was to investigate the potential therapeutic effect of inhibiting the integrated stress response with ISRIB (integrated stress response inhibitor) at chronic timepoints after traumatic brain injury. DATA COLLECTED: The dataset includes several experiments with 152 male C57BL/6J (RRID:IMSR_JAX:000664) mice using the parietal controlled cortical impact (CCI) or Closed Head Injury (CHI) TBI models. A summary of experiments is as follows: (1) Ratio of phosphorylated eIF2a to total eIF2a protein levels between Sham and TBI animals as quantified by quantitative western blot analysis at acute (1 days post injury; dpi) and chronic (26-28 dpi) timepoints. (2) Performance of Sham and CCI animals treated with Vehicle or ISRIB (2.5mg/kg ISRIB) on radial arm water maze (errors; RAWM). Treatment was given starting on the day prior to behavior and after the final trial of each day of behavior. (3) Performance of Sham and CHI animals treated with Vehicle or ISRIB (2.5mg/kg ISRIB) on delayed-matching-to-place Barnes maze (time to escape; DMP). Treatment was given starting on the day prior to behavior and after the final trial of each day of behavior. DATA USAGE NOTES: