2D trajectories of human pluripotent stem cell colonies

This dataset is a part of the following manuscript submitted for publication in Life (MDPI):

"Human Pluripotent Stem Cell Colony Migration is Related to Culture Environment and Morphological Phenotype"by Vitaly V. Gursky, Alina S. Chabina, Olga A. Krasnova, Anastasiya A. Kovaleva, Daria V. Kriger, Michail S. Zadorsky, Konstantin N. Kozlov, and Irina E. Neganova

A trajectory in the csv file is an array of the 2D coordinates {{x1,y1}, {x2,y2}, ...} (in µm; centered at {x,y}={0,0}), which represent the migration history of the center of one cell colony. Trajectories were obtained from the time-lapse bright-field images, using the Manual Tracking tool in ImageJ (Fiji). The data were collected for colonies from three human pluripotent stem cell lines (human induced pluripotent stem cell line AD3, patient specific human induced pluripotent stem cell line HPCASRi002-A (CaSR), and human embryonic stem cell line H9), grown under two culture conditions (media/matrix = mTESR1/MG or E8/GT). The colonies were phenotyped according to their morphological characteristics associated with the pluripotency status ("good" and "bad" phenotype); cells with the "bad" phenotype showed signs of incipient differentiation. The time interval between adjacent coordinates is 15 min.

The data were obtained under the financial supprot of the Russian Science Foundation, grant number 21-75-20132.