Transcriptional profiling on leaves of Pha13 (PATC148746) overexpressed Phalaenopsis aphrodite

To identify genes affected by Pha13, Pha13 was transiently overexpressed in leaves of P. aphrodite by agroinfiltration. The leaves of P. aphrodite infiltrated with agrobacterium carrying empty vector (without Pha13) was used as a control. Transcriptional profiling was analyzed using a customized oligonucleotide array with RNA extracted from Pha13 and empty vector overexpressed leaves of P. aphrodite. The sequences used for probe design were collected from the Orchidstra 2.0 database (http://orchidstra2.abrc.sinica.edu.tw/orchidstra2/index.php). Totally, 42,973 probes were designed on this oligonucleotide array. Two-condition experiment, Empty vector (EV) vs. Pha13 transient overexpression (Pha13-OE). Biological replicates: 3 control replicates, 3 transient overexpression replicates.