Additional file 1: Figure S1. of Evolutionary comparison of prenylation pathway in kinetoplastid Leishmania and its sister Leptomonas

Multiple sequence alignment of FTase (α-subunit) of Leishmania donovani (accession number XP_003862625.1, putative protein) with FTase (α-subunit) of Leishmania major (accession number XP_003722277.1, putative protein), Leishmania infantum (accession number XP_001466722.1, putative protein) and its sister Leptomonas (contig_2654, putative protein) showing conserved regions (red colored) based on percentage identity. Figure S2. Multiple sequence alignment of FTase (β-subunit) of Leishmania donovani (accession number XP_003861732.1) with FTase (β-subunit) of Leishmania major (accession number XP_001684151), Leishmania infantum (accession number XP_001470492) and its sister Leptomonas (contig_1135) showing conserved regions (red colored) based on percentage identity. Figure S3. Multiple sequence alignment of GGTase-II (α-subunit) of Leishmania donovani (accession number XM_001468149) with GGTase-II (α-subunit) of Leishmania major (accession number XM_001685808) and Leishmania infantum (accession number XP_001468186) showing conserved regions (red colored) based on percentage identity. Its sister Leptomonas has no α -subunit of GGTase-II. Figure S4. Multiple sequence alignment of GGTase-II (β-subunit) of Leishmania donovani (accession number XP_003864545) with GGTase-II (β-subunit) of Leishmania major (accession number XP_001686510) and Leishmania infantum (accession number XP_001468743) showing conserved regions (red colored) based on percentage identity. Figure S5. Alignment of various Leishmania donovani Rab protein sequences showing sequence similarities and domain conservation among them colored red based in their percentage similarity. Figure S6. Alignment of various Leptomonas Rab protein sequences showing sequence similarities and domain conservation among them colored red based in their percentage similarity. Figure S7. Phylogenetic relationship among Rab sequences of L. major, L. infantum, L. donovani and Leptomonas which is based on multiple sequence alignment (ClustalW algorithm in MEGALIGN with 1000 bootstraps). Figure S8. Circos plot was generated using Circos version 0.60, which is used to determine the similarity between Rab genes of Leishmania donovani and its sister Leptomonas. Where cyan color for all Rabs protein obtained from Leptomonas genome and green color for all Rabs protein obtained from Leishmania donovani genome. Other colors for each different Rabs. (ZIP 81526 kb)