Two complete mitochondrial genomes of <i>Boulenophrys</i> (Anura: Megophryidae: Megophryinae): characteristics and phylogenetic implications

The Chinese horned toads, <i>Boulenophrys boettgeri</i> (Boulenger, 1899) and <i>Boulenophrys kuatunensis</i> (Pope, 1929), are two captivating species within the family Megophryidae, which inhabit the mountainous streams in the Eastern of China. In this study, two new complete mitochondrial genomes of <i>B. boettgeri</i> and <i>B. kuatunensis</i> were sequenced, assembled, and annotated using next-generation sequencing. The length of mitochondrial genomes of <i>B. boettgeri</i> and <i>B. kuatunensis</i> was 16,597 and 17,921 bp, respectively, with both containing 13 protein coding genes, 22 tRNA genes, two rRNA genes, and one putative control region. Phylogenetic relationships based on protein-coding mitochondrial genes showed that the two <i>Boulenophrys</i> species formed a cluster with other <i>Boulenophrys</i> species. The two new sequences provide valuable insights into the mitochondrial genomes of these two species, offering important data for understanding the phylogenetic relationships of the genus <i>Boulenophrys</i>.