Specific enrichment of H2A.Z.1 or H2A.Z.2 in the gene body of genes in Caco-2/15 subconfluent cells.

ChIP-seq experiments with Flag antibody have been done in Caco-2/15 cell lines expressing Flag-tagge H2A.Z.1 or H2A.Z.2 histone variant isoforms. Genomic occupancy of both isoforms was meseasured from subconfluent (Sc) cells, the cell populations expressing H2A.Z.1-flag being called “Z1Sc”, whereas that expressing H2A.Z.2-flag are named “Z2Sc”. We then computed for each gene the number of reads present in TSS-associated peaks or gene body-associated peaks. Interestingly, a significant population of genes (2516 genes for H2A.Z.1 and 2542 genes for H2A.Z.2) have more signal in peaks in their body than at their TSS in subconfluent cells. The lists of these genes are shown in this file.本数据集针对表达Flag标签化H2A.Z.1或H2A.Z.2组蛋白变异体亚型的Caco-2/15细胞系，采用Flag抗体开展了染色质免疫共沉淀测序（ChIP-seq）实验。研究人员从亚汇合（Sc）状态的两类细胞群中分别测定了两种组蛋白变体的基因组结合分布：表达H2A.Z.1-Flag的细胞群命名为"Z1Sc"，表达H2A.Z.2-Flag的细胞群则命名为"Z2Sc"。随后，我们针对每个基因，统计了其转录起始位点（Transcription Start Site，TSS）相关峰以及基因本体区域相关峰内的测序读段数目。值得注意的是，存在显著数量的基因（H2A.Z.1组对应2516个基因，H2A.Z.2组对应2542个基因）在亚汇合状态细胞中，其基因本体区域峰的信号强度高于转录起始位点区域峰。上述两类基因的列表均收录于本文件中。