Intestinal flora date-Group_NCD_HFD_LFBE.zip

All high-quality sequencing reads were clustered using USEARCH (v7.0.1090) with 97% similarity, and operational taxonomic units (OTUs) were obtained with a certain threshold. Each OTU was selected as the representative sequence and subjected to RDP Classifier software (v2.2) for taxonomical assignment. The number of sequences per sample was corrected for differences in sequencing depth between samples by rarefication, that is, the same number of reads is randomly subsampled in each sample. Second, the absolute number of sequences of each OTU in each sample was converted to relative abundance to reduce the effect of differences in sequence reads. The representative sequences, together with the abundance data, were used for taxonbased analysis.