Preventive Effect of Lactobacillus Mixture on Inflammation and Cytokines in Lipopolysaccaride-Induced Cystitis Model

Severity of bladder inflammation was categorized according to the BII proposed by Jerde et al., with consideration of leukocyte infiltration into lamina propria and interstitial tissue edema. Leukocyte infiltration was assessed in 1mm² areas of tissue section. Score was 0 when there was no infiltration, 1 when there were less than 20 leukocytes, 2 with 20-45 leukocytes, and 3 when there were over 45 leukocytes. With regards to edema in the interstitial tissue, a score of 0 was given for no edema; 1 for mild edema, thinner than the mucosa; 2 for moderate edema, thinner than twice the thickness of the mucosa; and 3 for severe edema, thicker than twice the thickness of the mucosa. Scores for leukocyte infiltration and interstitial edema were added and the average scores for three sites (bladder neck, lateral wall, and bladder dome) in each of the bladder specimens were compared.