RNA-Seq analysis of gene expression profile of human monocytes infected by Vaccinia Virus

In this study, we employed high throughput sequencing (RNA-Seq) to investigate the gene expression profiles in THP-1 (monocyte cells )before and post-infection of VACV. we conducted RNA-Seq and analyzed differentially expressed genes in THP-1 cells with and without VAVC infection, and then performed the enrichment analysis, function annotation, and STRING network analysis. 4976 genes with significant differential expression (2416 were up-regulated and 2380 were down-regulated). Further KEGG enrichment analysis suggested that 27 significant signaling pathways, including metabolism, immune inflammation regulation, cell function regulation, cancer and myocardial disease, pathogen infection, were affected by VACV infection. Gene Ontology (GO) functional annotation indicated that these genes were mainly enriched on cell level regulation, immunological processes, protein levels, and signal transduction pathway. The STRING online database analysis showed that JUN,CHUK,IL1B,PYCARD protein were higher in the infected THP-1 cells. RNA-seq of Vaccinia virus treatment human monocytes at the indicated stages were generated by deep sequencing, 3 replicates per samplein duplicate, using NovaSeq platform.RIP-seq of human monocytes were generated by deep sequencing, 3 replicates per samplein duplicate, using NovaSeq platform.MeRIP-Seq of human monocytes at the indicated stages were generated by deep sequencing, 3 replicates per samplein, using illumina Hiseq instrument.