RNAseq for calyx development in Dipterocarpaceae

The goal of this project is to understand the regulation of calyx development in the tropical woody family Dipterocarpaceae. To identify genes responsible for the regulation of calyx development in this family, we performed RNAseq using calyx samples obtained from 12 dipterocarp species. The collected calyxes were submerged in RNAlater immediately after harvesting. Total RNA was extracted from each sample using the CTAB method. Sequencing libraries were constructed for the 16 RNA samples obtained separately. The libraries were sequenced using the Illumination HiSeq 2000 platform, to generate 100 bp paired-end reads.