Georeferenced phylogenetic analysis of a global collection of wild and cultivated Citrullus species

We used 135 accessions of fCitrullus spp. to generate sequence data based on chloroplast regions. Two regions of the chloroplast genome were amplified using the following primer pairs: 1) ndhF (5'-GAAAGGTATKATCAAYGMATATT-3') and rpl32-R (5'-CCAATATCCCTTYYTTTTCCAA-3'); and 2) trnL(UAG) (5'-CTGCTTCCTAAGAGCAGCCT-3') and trnT(GGU) (5'-CCCTTTTAACTCAGTGGTAG-3'). Parsimony networks were constructed to infer phylogeographical relationships among haplotypes using TCS v1.21 (Clément et al., 2000).