FACS-sorted GFP-positive ureteric bud (UB) cells at postnatal day 0 (P0): wildtype (WT) vs. conditional Dot1-knockout (KO) from Hoxb7-positive cells

Transcriptional profiling of GFP-positive UB cells from Hoxb7CreGFP mice without (WT) or with (KO) homozygously floxed DOT1 alleles at the age of postnatal 0. This experiment aimed to uncover the genome-wide alternation in gene expression resulting from the removal of DOT1 gene in the collecting duct cell population (Hoxb7 positive) and successive changes to the series of events in kidney development. Two-condition experiment, Dot1 WT vs. KO UB cells. Biological replicates: 4 WTs and 4 KOs.