Characterization and comparison of intestinal bacterial microbiomes of Euschistus heros and Piezodorus guildinii collected in Brazil and the United State

Herbaceous insects are one of the main biological threats to crops. One such group 22 of insects, stink bugs, do not eat large amounts of tissue when feeding on soybean, but are 23 extremely damaging to the quality of the seed yield as they feed directly on green developing 24 seeds leading to poorly marketable harvests. In addition to causing physical damage to the seed 25 during feeding, the insects can also transmit microbial pathogens, leading to even greater yield 26 loss. Conducting surveys of the insect intestinal microbiome can help identify possible 27 pathogens, as well as detail what healthy stink bug digestive systems have in common. We used the conserved V4 region of the 16S rRNA gene to characterize the bacterial 29 microbiome of the red-banded stink bug Piezodorus guildinii collected in Brazil and the United 30 States, as well as the neotropical brown stink bug Euschistus heros collected in Brazil.