Bos taurus breed:Holstein Transcriptome or Gene expression

Johne's disease is a chronic infection of the intestinal mucosa caused by Mycobacterium avium subspecies paratuberculosis (MAP). The events of pathogen survival, inflammation and progression to the clinical stages of infection are poorly understood. This study examines gene expression in the ileoceocal valve (ICV) of Holstein dairy cows at different stages of MAP infection. The ICV is known to be a primary site of MAP colonization and provides an ideal location to identify genes that are relevant to the progression of this disease. RNA was prepared from ICV tissues and RNA-Seq was used to compare gene transcription between clinical, subclinical, and negative control animals.