Phagocytic microscopy and MP-TFM assay with RAW macrophages upon treatment with cytoskeletal inhibitors

Confocal image stacks of fixed RAW macrophages phagocytosing soft 1.4 kPa DAAM-particles functionalized with IgG to trigger phagocytosis, and with AF488-Cadaverine for visualization. Cells were stained for F-actin, and particles were stained with a fluoresecent secondary antibody to reveal the exposed surface. Cells were treated with myosin-II inhibitor Blebbistatin, Arp2/3 inhibitor CK666 or formin inhibitor SMIFH2 prior to and during the phagocytic assay. Control cells were treated with DMSO. File Names contain DMSO, Bleb, CK666 or SMIFH2 to indicate the treatment group.