Effect of amphipaths on cholesterol dynamics in fixed fibroblasts.
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Cells were suspended, fixed, washed and preincubated for ∼5 min at 37°C with 0.05 mM CPZ or 0.8 mM octanol. Cholesterol oxidase was then added to initiate time courses which were analyzed as described in Method B of Materials and Methods and illustrated in Figure 4. Values are means ± average deviations for 2 independent experiments or means ± SD for n>2.
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2015-12-02



