Analysis of pROCK parasite DNA lesions compared with MutT parasites through a QPCR assay.

Specific primers were used to amplify large and small fragments of the nuclear and mitochondrial DNA of untreated cells. Normalized amplification fluorescence values are in Table 1. This values were used to estimate the relative amplification of pROCK cells compared to MutT. The pROCK average number of lesions per 10 kb of the genome in relation to MutT parasites was then estimated by the relative amplification calculated (*** P<0.0001, unpaired t test).