CMV-specific cytotoxic T lymphocytes (CTLs) RNA-seq at the late phase of allo-HCT. CMV-specific cytotoxic T lymphocytes (CTLs) RNA-seq at the late phase of allo-HCT

We conducted immune- and RNA-sequencing of HLA-A24-restricted CMVpp65-specific CTLs to better understand the immune reconstitution of CMV-CTLs after allo-HCT. To the best of our knowledge, this is the first report on the features of TCRβ-CDR3, diversity, and GEP of HLA-A24 CMV-CTLs according to the CMV-reactivation pattern among recipients after allo-HCT. In addition, we further sought to demonstrate homogeneity or heterogeneity according to individual CTL clones using single-cell RNA-sequencing technology. Overall design: Compare GEP in CMV-CTLs according to the CMV-reactivation pattern and donor CMV serostatus at the late phase of allo-HCT.