RNA-seq (FPKM), RT-qPCR, and qPCR datasets of host responses in Orientia tsutsugamushi-infected mouse macrophages

This dataset contains gene expression and validation data derived from primary mouse macrophages infected with Orientia tsutsugamushi. The primary dataset consists of a genome-wide RNA-seq expression matrix reported as FPKM (Fragments Per Kilobase of transcript per Million mapped reads) values for 28,000+ genes, aligned to the Mus musculus reference genome (mm10). The matrix includes genomic annotations (gene ID, chromosome, genomic coordinates) and normalized expression values across multiple experimental conditions and biological replicates.
In addition to the RNA-seq dataset, the file includes qRT-PCR validation data for selected host genes (Figures 1, 4, and 5), with expression levels normalized to Gapdh. The dataset also contains bacterial burden measurements quantified by qPCR targeting the 47-kDa gene, expressed as gene copy number per sample.
Together, these data provide both transcriptome-wide expression profiles (FPKM) and targeted validation of host response and bacterial load under different infection conditions.