Quantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single base resolution. Quantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single nucleotide resolution

To investigate the genomic levels of 5-hydroxymethylcytosine at single-base resolution. The current study developed a method which allows one to study hydroxymethylation of cytosines in the genome via a subtractive method of RRBS and oxidative RRBS.