Transcriptional analysis of primary adult human skin cells that express stage-specific embryonic antigen 3 (SSEA-3). Homo sapiens

Primary human skin cells were gated based on expression of stage-specific embryonic antigen 3 (SSEA3) with the high SSEA3 expressing cells (top 10%, categorized as SSEA3-positive or SSEA3-high) and negative SSEA3 expressing cells (bottom 10%, categorized as SSEA3-negative) purified into two different subpopulations. Sorted cells were allowed to adhere overnight under DMEM/F12 + 10% FBS culture conditions before total RNA was collected and global transcriptional analysis was performed. Overall design: We isolated primary adult human skin cells with either high SSEA3 expression or negative SSEA3 expression via FACS and analyzed via Affymetrix microarray analysis.