Transcriptomic Analysis of Mycobacterial Infected Macrophages at Different MOIs

Macrophage infection models are important tools for studying host-mycobacterial interactions. Although the multiplicity of infection (MOI) serves as an important experimental variable, the selection of MOI in mycobacterial infection experiments is largely empirical, without reference to solid experimental data. To provide relevant data, we used RNA-seq technology to analyze the gene expression profiles of macrophages (MP) 4 or 24 hours after infection with Mycobacterium marinum (M. m) at MOI's ranging from 0.1 to 50.