UV-VIS Spectra Activated Droplet Sorting for Label-Free Chemical Identification and Collection in Droplets

Data underlying the figures in the publication “UV-VIS Spectra Activated Droplet Sorting for Label-Free Chemical Identification and Collection in Droplets”, published in <em>Analytical Chemistry, </em><strong>2021</strong>. Table of contents: <strong>1. Fig2_BSA_calibration_2000us</strong>; Excel file containing the BSA UV-VIS spectra corresponding to <em>Figure 2</em>. It demonstrates the calibration of the UV-VIS in-droplet measurement. Spectroscopy settings: 2000 us integration time and 200 nm to 800 nm wavelength range. Samples: PBS (control), 500 uM BSA, 250 uM BSA, 100 uM BSA, 50 uM BSA, and 10 uM BSA. <strong>2. Fig3_Data</strong>; Excel file containing the UV-VIS time-lapse data for high-throughput droplet measurements used in all subfigures of <em>Figure 3</em>. <em>Fig3data.csv</em> is a semi-colon separated csv file for UV-VIS measurements collected over ~30 minutes. Saved wavelengths include 250, 280, 300, 311, 315, 400, 500, and 600 nm. An integration time for the spectrometer of 500 us was used. <strong>3. Fig4_C_Postsorting and Fig4_C_Presorting</strong>; Overlaid bright field &amp; fluorescent images of droplets containing E. coli expressing gfp microcolonies before and after droplet sorting. <strong>4. Fig4ABD_Data</strong>; Zip. archive containing the experimental data for <em>Figure 4</em>. Interaction of polymersome-based melanosome mimics with HaCaT cells. (A) Cell proliferation assay showing the non-toxicity of different polymersomes encapsulating dopamine and Tyrosinase (dashed purple), L-DOPA and <strong>5. Fig5A_Combined Data</strong>; Excel file containing the UV-VIS spectra for <em>Figure 5A</em>. Separate sheets contain spectra for the 3 conditions: empty droplet, mutant (ergothionase activity negative) droplets, and wild type (positive) droplets. UV-VIS range from 200 nm to 1000 nm. <strong>6. Fig5B_Ergothionase assay_wild type mutant mix</strong>; Excel file containing the UV-VIS time-lapse data for high-throughput droplet measurements used in <em>Figure 5B</em>. “Fig5B_Ergothionase assay_wild type mutant mix.csv” is a semi-colon separated csv file. Wavelengths 280, 311, 400, and 600 nm are saved. An integration time of 2000 ms was used. “Fig5B_Ergothionase assay_wild type mutant mix_settings.conf” is a configuration file for the spectrophotometer used for this measurement. It describes all spectrometer settings used in the droplet sorting process. <strong>7. Fig5C_Combined Data</strong>; Excel file containing the 311 nm absorbance data of <em>Figure 5C</em>; from droplets before and after droplet sorting. The data from both conditions are separated as sheets within the file. An integration time of 2000 ms was used for these measurements. <strong>8. SI_Absorbance_by_Geometry</strong>; Excel file containing the data used in <em>Figure S1</em> to test different UV-VIS detection region geometries.