Single cell hierarchy of the mouse vaginal epithelium. Single cell hierarchy of the mouse vaginal epithelium

In this study, using genetic cell lineage tracing, vaginal organoids, targeted gene deletion and single cell sequencing approaches; we revealed a unique population of vaginal epithelial stem cells and provided evidence that canonical Wnt/b-catenin signaling is required for the proliferation and differentiation of these stem cells. Overall design: Mice vaginae of Wild Type (WT) or Ovarectomised (OVX) C57BL/6 mice were analysed using Dropseq. Each group in the dataset was produced by pooled samples of 5 individuals and run in duolicate (i.e. N701 WT1 (5 mice pooled); N702 WT2 (5 mice pooled); N703 OVX1 (5 mice pooled) and N704 OVX2 (5 mice pooled))