VEGF-Overexpression

Human dermal fibroblasts (Hu02) were transfected by pcDNA3.1(-)-VEGF vector. Following selecting fibroblast cells with hygromycin, recombinant cells were investigated in terms of VEGF expression by quantifying method. We used Real-Time PCR assay to quantitate gene expression of vascular endothelial growth factor from manipulated cells and represented VEGF overexpression.将pcDNA3.1(-)-VEGF载体转染人类皮肤成纤维细胞株Hu02。经潮霉素筛选获得阳性成纤维细胞后，采用定量方法对重组细胞的VEGF表达情况进行检测；本研究通过实时荧光定量PCR（Real-Time PCR）实验，对转染后细胞的血管内皮生长因子（VEGF）基因表达水平进行定量分析，证实了VEGF的过表达。