Additional file 2 of Phenotypic screen and transcriptomics approach complement each other in functional genomics of defensive stink gland physiology

Additional file 2: Supplementary Table S2. Gene ontology of iBeetle-identified genes affecting stink function (separate, sortable Excel file). BLASTp against the Tribolium genome assembly Tcas5.2 [50] was used to identified the respective beetle genes and tBLASTn was used against the D. melanogaster genome and the entire NCBI nucleotide collection database. In addition, gene ontology analyses were carried out using ShinyGO [44], BlastKOALA [45], and eggNOG-mapper [46]. In ShinyGO, n.a. specifies that one gene (iB-02716) was not analyzed, whereas * indicates the four genes (iB-02516, iB-05119, iB-09043, iB-09239) involved in lysosome function. In BlastKOALA, 24 genes were not analyzed, while in eggNOG-mapper only for four genes (iB-03552, iB-04420, iB-07361, iB-08861) no orthologs were identified. In eggNOG, for one gene (iB_04702) the closest seed ortholog was from the African clawed frog (Xenopus laevis)**, while for one gene (iB_08184) it was from the purple sea urchin (Stronglylocentrotus purpuratus)***. For all other genes, T.castaneum genes were used as seed orthologs by eggNOGG, with 13 genes identified by former Tc-numbers (indicated in column C in addition).