CLT-seq as a universal homopolymer-sequencing concept reveals poly(A) tail-tuned ncRNA regulation

We describe central limit theorem (CLT)-managed RNA-seq (CLT-seq) as a universal homopolymer-sequencing concept that features unbiased RNA-seq and facilitates accurate poly(A)-tail base-calling. CLT-seq delivers high-quality poly(A)-tail profiling at a transcriptome-wide scale in human cellular contexts. We find that poly(A) tail-tuned ncRNA regulation undergoes a dynamic complex process as mRNA. An endogenous poly(A)-tail profile-correlation pattern enables the reliable mapping and cataloging of all cell types.