Supplemental Material for Caraballo et al., 2020

<b>Supplementary Figure 1. </b>Branch-specific relaxation in <i>Caenorhabditis </i>UGGTs recognition (top) and catalytic (bottom) domains. Branches are color-coded according to the selection strength parameter k, inferred under the general descriptive RELAX model. Low k values (&lt;1) indicate purifying selection relaxation, whereas high k values (&gt;1) indicate selection intensification.<b>Supplementary Figure 2. </b>Branch-specific relaxation in vertebrate UGGTs recognition (left) and catalytic (right) domains. Branches are color-coded according to the selection strength parameter k, inferred under the general descriptive RELAX model. Low k values (&lt;1) indicate purifying selection relaxation, whereas high k values (&gt;1) indicate selection intensification. <b>Supplementary Figure 3 (in Additional File 5).</b> Expression of <i>Sp</i>UGGT, <i>Ce</i>-UGGT-a, <i>Ce</i>-UGGT-b and chimeric proteins in <i>alg6/gpt1- </i>cells. Microsomal proteins derived of <i>S.pombe alg6/gpt1 </i>transformed with pREP3X-<i>uggt</i>-b (lane1), pREP3X-<i>uggt</i>-a (lane 2), pREP3X-<i>gpt1+ </i>(lane 3), pREP3X-chimera I (<i>Ce</i>-N-term-<i>uggt</i>-a-C-term-<i>gpt1+c-myc</i>) (lane 4), pREP3X-chimera II (<i>Ce</i>-N-term-<i>uggt-b</i>-C-term<i> gpt1+</i>-<i>c-myc</i>) (lane 5) and pREP3X (lane 6) were analyzed in 8 % SDS PAGE and submitted to Western-blot analysis using a commercial anti c-Myc antibody. <b>Table S1 </b><b>(in Additional File 5)</b><b>.</b> Templates and primers used in the PCR amplification of the sequences encoding the N- and C-terminal domains of <i>S. pombe</i> UGGT, <i>Ce</i>-UGGT-a, and <i>Ce</i>-UGGT-b.<b> Table S2 </b><b>(in Additional File 5)</b><b>.</b> Templates and primers used in the PCR amplification of the full-length fragments encoding chimeric UGGTs <b> Table S3 </b><b>(in Additional File 5)</b><b>.</b> List of DNA primers used in this work<b>Additional File 1</b>. <b>(xls). </b>Species names, phyla, database and Accession Numbers of the 195 UGGT (and UGGT-like) sequences included in this study. The criteria for excluded sequences is detailed.<b> Additional File 2. (fas).</b> Unaligned 195 UGGT (and UGGT-like) protein sequences.<b> Additional File 3. (fas).</b> Alignment of 195 UGGT (and UGGT-like) protein sequences used for phylogenetic inference.<b> Additional File 4. (doc).</b> Aminoacidic positions corresponding to each UGGT domain taken as reference sequences. <b> Additional File 5. (doc).</b> Procedures performed in the construction of expression plasmids and list of primer sequences used in this study. Supplementary Figure S3. Western-blot analysis of the expression of the c-Myc labeled full length and chimeric proteins.<br>