Induction of ER stress in SHSY5Y human neuroblastoma cells by thapsigargin treatment

To identify the impact of ER stress on other cellular organelles, cells were treated with ER stress inducer thapsigargin. Transcriptomic profile revealed increased ER stress levels. Deregulated genes was further screened to identify novel ER stress markers of other organelles. Functional charecterization of novel markers were performed to gain insight of the observation. Overall design: SHSY5Y human neuroblastoma cells were treated with thapsigargin with 0.5 µ? conc. for 3hrs. RNA isolation was carried out by TRIzol method followed by RNA sequencing. Differential gene expression analysis was performed to identify the deregulated genes under thapsigargin mediated ER stress.