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BR-body mutant mRNA half-life profiling.

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https://www.ncbi.nlm.nih.gov/sra/SRP212634
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BR-body mutant strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. JS38 (wild type) was compared to JS221 (lacking the intrinsically disordered CTD and unable to form BR-bodies), JS233 (unable to recruit degradosome components into BR-bodies), and JS299 (active site mutant of RNase E). Overall design: BR-body mutant strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. Biological samples were taken before treatment, then at 1, 3, and 9 minutes after rifampicin treatment.
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2019-07-08
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