Whole genome gene expression profiles of migratory cells in the Drosophila ovary. Drosophila melanogaster

Cell migration contributes to normal development and homeostasis as well as to pathological processes such as inflammation and tumor metastasis. Previous genetic screens have revealed a few major signaling pathways that govern follicle cell migrations in the Drosophila ovary, several of which elicit transcriptional responses. However few downstream targets of the critical transcriptional regulators, such as the C/EBP homolog SLBO, have been identified. To characterize the gene expression profile of two migratory cell populations and identify SLBO targets, we employed a magnetic bead based cell separation approach to purify border cells and centripetal cells expressing the mouse CD8 antigen, and carried out whole genome microarray analysis. Keywords: cell type comparison, genetic modification Overall design: 12 samples were analyzed to compare gene expression between different cell types or gene types. Three biological repeats were done for each experiments.