Multiregional single-cell profiling reveals extensive field cancerization and an immunosuppressive microenvironment in oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) is highly heterogeneous and metastatic, and the mechanisms driving OSCC development, progression, and metastasis are poorly understood. We performed multiregional single-cell RNA sequencing on 268,131 cells obtained from tumor core, tumor periphery, adjacent non-tumor tissue, and metastatic lymph node samples from 10 patients with human papillomavirus (HPV)–negative metastatic OSCC. Single-cell RNA-seq: 10X data of 36 samples from 10 OSCC patients