Microbiota profiling of Hyalomma marginatum ticks using 16S rRNA amplicon sequencing in Corum, Turkey

This project aims to characterize the bacterial community structure associated with Hyalomma marginatum tick pools collected from Corum, Turkey, using 16S rRNA gene amplicon sequencing targeting the V3-V4 region. Tick samples were processed in pooled groups, and genomic DNA was extracted and subjected to Illumina-based next-generation sequencing. Bioinformatics analyses were performed using QIIME2, including quality filtering, denoising with DADA2, and taxonomic assignment based on the SILVA 138 reference database. This dataset provides insights into the microbiota composition of Hyalomma marginatum and contributes to understanding microbial diversity associated with tick vectors.