five

Primers and PCR conditions used for amplification of seven overlapping fragments (5′NCR-prM, E, E-NS3, NS3, NS3-NS5, NS4B-NS5, and NS5-3′NCR) to obtain complete genomic sequence.

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https://figshare.com/articles/dataset/_Primers_and_PCR_conditions_used_for_amplification_of_seven_overlapping_fragments_5_8242_NCR_prM_E_E_NS3_NS3_NS3_NS5_NS4B_NS5_and_NS5_3_8242_NCR_to_obtain_complete_genomic_sequence_/1117609
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All the primers – outer forward (OF), outer reverse (OR), inner forward (IF), and inner reverse (IR) were identical for Saringe 2009 - and Mandal 2009 except E-NS3 OF. The nucleotide positions (nt) are related to the strain Toro 2003.
创建时间:
2014-07-24
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