Transcriptome analysis of Sinorhizobium meliloti strain ectopically overexpressing trpL

We show that the RNA leves of many genes in the S. meliloti overexpressing strain (OE) 2011 (pRK-RcsR1) are more similar to the levels of in wild type (WT) strain 2011 than in the empty vector control (EVC) strain 2011(pRK4352). Since the plasmid-containing strains were grown in the presence of 10 µg/ml tetracycline (Tc), while the WT was grown without Tc, the results suggest that the intrecellular Tc concentration in the OE strain are lower than in the EVC. This is explained by an increased efflux pump activity in the OE strain. Overall design: Total RNA of the three strains was subjected to RNAseq analysis (Illumina) contributor: vertis Biotechnologie AG (Munich, Germany)