Summary of TaqMan miRNA qPCR, DNA PCR and p16 staining results, as well HPV detection and/or genotyping results by LDR, Luminex and Hybrid Capture 2.

Obtained positive results in TaqMan qPCR out of performed runs are given. HPV6-miR-H1 did not give any positive results. Positive signals were obtained for HPV16-miR-H1, HPV16-miR-H2, HPV38-miR-H1 and HPV68-miR-H1 from cell lines and patient samples. Positive control U6 gives positive result in every reaction. The presence of pre-miRNA coding regions for HPV16-miR-H1 and HPV16-miR-H2 was confirmed by DNA PCR in all cell lines and many tissue samples. Results of p16 tissue staining, as well as results of HPV genotyping by LDR or Luminex, and high risk HPV detection by Hybrid Capture 2 are given. NA, not analyzed. ND, not done (inadequate sample). CIN1-3, cervical intraepithelial neoplasia 1–3; Cond pl, condyloma planum; SCC, squamous cell carcinoma; AIS, adenocarcinoma in situ; AC, adenocarcinoma; SE, squamous epithelium; CE, columnar epithelium; LDR, ligase detection reaction based HPV genotyping assay; Luminex, Luminex based HPV genotyping assay; HC2, Hybrid Capture 2 HPV detection assay; *, numbers are HPV types; NA, not analyzed; ND, no data (not enough material for testing).