Gene expression profiles of mesenchymal stromal cells in MDS mice.

To characterize the dysregulated MSCs in the murine MDS models, we performed a single-cell RNA sequence (scRNA-seq) analysis of FACS-sorted MSCs. The proportions of subclusters comprising MSCs were discrete between controls and MDS, and Osteolineage population was reduced in MDS compared with the control. In addition, Lepr+ mesenchymal population in MDS mice exhibited the remarkable reduction of skeletal stem marker Grem1 and MSC marker genes. This transcriptome analysis suggested that the osteolineage differentiation of MSCs is suppressed in vivo in the presence of MDS cells. Overall design: Experiments using MSCs from two MDS model mice, Nup98-HoxD13 (WT_A1 and Tg_A2) and Abcg2(Control and Abcg2).