Mytilus chilensis Raw sequence reads 16S

A next-generation sequencing (NGS) approach was used to study the microbiota associated to Mytilus chilensis gut, applying pyrosequencing of PCR-amplified V6-V8 16S rDNA gene regions. We analyzed the gut resident bacterial communities in individuals collected from two locations, individuals free living in natural conditions (Wild Type group, WT) and individuals collected form intensive commercial cultures in Mussels Farms (MF group) in the Chilean coast. DNA samples were amplified, and quality-filtered reads were assigned to family and genus taxonomic levels using the DADA2 pipeline. A total of 1,2 M of non-chimeric sequences were detected, belonging to 30 phyla, including Fusobacteria, Proteobacteria, Bacteroides, Tenericutes, and 404 genera taxas. The major fraction of the sequences detected corresponded to Vibrionaceae and Fusobacteriaceae families for MF and WT groups, respectively. The microbiota of M. chilensis gut have a wide diversity, however differences were observed according to the origin of the samples, where gut samples from mussel farm show a larger number of genera compared to gut tissue samples collected from wild type individuals. The dominant bacterial genera appeared to be Vibrio, Psychrilyobacter, Mycoplasma and Psychromonas, that were present among the five samples analyzed with different relative abundancies. This next generation sequencing technique, applied for the first time in Chilean mussel (Mytilus chilensis), show that the gut is a useful tissue for the study of the bacterial communities in this bivalve mollusk. The results obtained in this study provide insights about the composition of the microbiota of M. chilensis unravelling the great bacterial diversity and the habitat effect on the bacterial community structure.