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Genome-wide measurement of RNA levels following depletion of RNAPII from the nucleus, in wildtype and SPP1 deletion strains.

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Ablation of H3K4me3 by deletion of SPP1 has no strong or consistent effect on the rate of mRNA degradation following depeletion of RNAPII from the nucleus. Overall design: Comparison of the rate of mRNA transcript degradation in strains with or without SPP1 following RNAPII depletion from the nucleus via an anchor-away system, determined by RNA-seq.

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2019-07-08
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