Effect of acute systemic inflammation on the Cerebral Vasculature in mice

To capture the global gene expression patterns in the cerebral vasculature following acute systemic inflammation, we injected the mice with LPS and sacrificed them after 15 min, 30 min, and 4 hrs, followed by the isolation of the cerebral vessels. RNA was isolated from the vessel lysates, followed by library preparation and RNA sequencing. Overall design: mRNA profiles of cerebral vessels isolated from the brains of mice (C57BL6, Wild type, 2-3 months old) injected (intra peritoneal) with 10mg/kg LPS for 15 min, 30 min and 4 hrs or PBS. (n=3 in each group, every sample in each group includes the cerebral vessels from one male and one female mice pooled together)